Tissue Factor Human Chromogenic Activity Assay Kit (ab108906)

製品の概要

  • 製品名
    Tissue Factor Human Chromogenic Activity Assay Kit
    Tissue Factor キット 製品一覧
  • サンプルの種類
    Urine, Serum, Plasma, Cell culture extracts, Tissue
  • アッセイタイプ
    Quantitative
  • 種交差性
    交差種: Human
  • 製品の概要

    The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. Tissue factor binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. Tissue factor markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates. Inducible expression of Tissue factor in a variety of pathological conditions, including gram-negative sepsis and acute coronary syndromes, is associated with life-threatening thrombosis. In sepsis, Tissue factor expression within the vasculature leads to disseminated intravascular coagulation. Tissue factor also plays important roles in vasculogenesis, metastasis, and tumor-associated angiogenesis.

     

    ab108906 Tissue Factor Human Chromogenic Activity Assay kit is developed to determine Human Tissue Factor activity in plasma, tissue and cell culture supernatants. The assay measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

画像

  • Colorimetric standard curve: mean of duplicates (+/- SD)

  • Standard curve showing concentration (pM) on X-axis and increase of O.D. per min on Y-axis (time frame: 20 min.)

  • Time course showing O.D.'s of one standard point and one sample (undiluted vs.diluted)

  • Tissue Factor measured in various biologicals showing average concentrations

プロトコール

参考文献

ab108906 has not yet been referenced specifically in any publications.

レビューと Q&A


The standard should be reconstituted in 3ml water.



After arrival please store the individual components as instructed in the protocol booklet:

ab108906 may be stored until the expiration date at 2 to 8°C from the date of shipment. Store Standard, Factor VII protein, Factor X pro...

Read More

Since tissue factor is both secreted and membrane bound, we recommend using cell lysates so the protein is properly released from the membrane.

As mentioned in the protocol under step 6.2) Cells lysates can be prepared as follows:

1. ...

Read More


The labs says that the kit contains a low binding plate which will be better. However, otherwise the brand of the 96-well plate does not matter.

Thank you for contacting us on Friday in regards to the Tissue Factor Human Chromogenic Activity Assay Kit (ab108906).

I can now inform you that the vial of Tissue Factor Standard you received required the addition of 1.2 ml of reagent grade...

Read More


The amount of standard is only provided on the vial label. The datasheet and the protocol bookletare only generic.
Please ask your customer tocheck the vial label, from which they can work out the volume of buffer they need to to generate a s...

Read More

Thank you very much for contacting us.
I just wanted to let you know that I am still working on your inquiry. I am looking forthe information you requested.
I will forward to you the information as soon as I receive it. Thank you for your pat...

Read More


I have just been informed that the tissue factor standard should be reconstituted with 1.2 ml of reagent grade water to generate a solution of 250 pM.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録