Rabbit polyclonal to Tissue Factor
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Tissue Factor.
(Peptide available as
This antibody gave a positive signal in Human plasma total protein tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Belongs to the tissue factor family.
Information by UniProt
CD142 antigen antibody
Coagulation factor III (thromboplastin tissue factor) antibody
Western blot - Tissue Factor antibody (ab85145)
Anti-Tissue Factor antibody (ab85145) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
50 kDa (
why is the actual band size different from the predicted?
Additional bands at:
148 kDa. We are unsure as to the identity of these extra bands.
The 50 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human Tissue factor precursor .
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"