製品の概要

  • 製品名Anti-Tissue Factor antibody
    Tissue Factor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tissue Factor
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 33 - 295 of Human Tissue Factor (BC011029).

  • ポジティブ・コントロール
    • WB: Human fetal kidney, brain, skin and lung lysates IHC-P: Human fetal kidney tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • 配列類似性Belongs to the tissue factor family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Anti-Tissue Factor antibody 画像

  • All lanes : Anti-Tissue Factor antibody (ab104513) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal skin lysate
    Lane 4 : Human fetal lung lysate


    Predicted band size : 33 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab104513, at a 1/100 dilution, staining Tissue Factor in formalin-fixed, paraffin-embedded Human fetal kidney tissue, showing membrane staining.

Anti-Tissue Factor antibody (ab104513) 使用論文

This product has been referenced in:
  • Cassidy MR  et al. Histone deacetylase inhibitors decrease intra-abdominal adhesions with one intraoperative dose by reducing peritoneal fibrin deposition pathways. Surgery 155:234-44 (2014). WB ; Rat . Read more (PubMed: 24239397) »
  • Darbousset R  et al. Tissue factor-positive neutrophils bind to injured endothelial wall and initiate thrombus formation. Blood 120:2133-43 (2012). Mouse . Read more (PubMed: 22837532) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - other (aortic vascular smooth muscle cells)
Specification aortic vascular smooth muscle cells
Treatment Indoxy sulfate 0.25-100 ug/ml
Blocking step Milk as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Dr. moshe shashar

Verified customer

投稿 Jul 18 2014

Thank you for contacting us.


I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
<...

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Thanks for the additional information.

I have initiated the request for a replacement of one vial of ab104513. Please contact me again if you have any further questions.

Thank you for submitting your completed questionnaire.

It does appear that this antibody is not working as expected. If you have received this antibody within the past 6 months or so I would be happy to offer a replacement.

In your ...

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Application Flow Cytometry
Sample Rat Cell (Plasma Microparticles)
Specification Plasma Microparticles
Preparation Cell harvesting/tissue preparation method: Centrifuge whole blood 1,500xg 15 min, then centrifuge the supernatant again at 13,000xg 2 min, and retain supernatant
Sample buffer: Annexin Binding Buffer
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy MP samples without label, and sample with secondary antibody only
Username

Abcam user community

Verified customer

投稿 Feb 23 2012

I am very pleased to hear you would like to accept our offer and test ab104513 in flow cytometry using rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forflow cyto...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"