製品の概要

  • 製品名
    Anti-Tissue Factor antibody
    Tissue Factor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tissue Factor
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 33 - 295 of Human Tissue Factor (BC011029).

  • ポジティブ・コントロール
    • WB: Human fetal kidney, brain, skin and lung lysates IHC-P: Human fetal kidney tissue

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/500.

ターゲット情報

Anti-Tissue Factor antibody 画像

  • All lanes : Anti-Tissue Factor antibody (ab104513) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal skin lysate
    Lane 4 : Human fetal lung lysate


    Predicted band size : 33 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab104513, at a 1/100 dilution, staining Tissue Factor in formalin-fixed, paraffin-embedded Human fetal kidney tissue, showing membrane staining.

Anti-Tissue Factor antibody (ab104513) 使用論文

This product has been referenced in:
  • Li Y  et al. Mechanism of the protective effects of the combined treatment with rhynchophylla total alkaloids and sinapine thiocyanate against a prothrombotic state caused by vascular endothelial cell inflammatory damage. Exp Ther Med 13:3081-3088 (2017). Read more (PubMed: 28587383) »
  • Abu El-Asrar AM  et al. Coexpression of heparanase activity, cathepsin L, tissue factor, tissue factor pathway inhibitor, and MMP-9 in proliferative diabetic retinopathy. Mol Vis 22:424-35 (2016). WB . Read more (PubMed: 27168718) »

See all 7 Publications for this product

Product Wall

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - other (aortic vascular smooth muscle cells)
Specification
aortic vascular smooth muscle cells
Treatment
Indoxy sulfate 0.25-100 ug/ml
Blocking step
Milk as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Dr. Moshe Shashar

Verified customer

投稿 Jul 18 2014

Thank you for contacting us.


I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
<...

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Thanks for the additional information.

I have initiated the request for a replacement of one vial of ab104513. Please contact me again if you have any further questions.

Thank you for submitting your completed questionnaire.

It does appear that this antibody is not working as expected. If you have received this antibody within the past 6 months or so I would be happy to offer a replacement.

In your ...

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Application
Flow Cytometry
Sample
Rat Cell (Plasma Microparticles)
Specification
Plasma Microparticles
Preparation
Cell harvesting/tissue preparation method: Centrifuge whole blood 1,500xg 15 min, then centrifuge the supernatant again at 13,000xg 2 min, and retain supernatant
Sample buffer: Annexin Binding Buffer
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
MP samples without label, and sample with secondary antibody only
Username

Abcam user community

Verified customer

投稿 Feb 23 2012

I am very pleased to hear you would like to accept our offer and test ab104513 in flow cytometry using rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forflow cyto...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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