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Purified human tissue factor apoprotein (47kD)
Our Abpromise guarantee covers the use of ab17375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab17375 staining Tissue Factor by Immunocytochemistry/ Immunofluorescence in mouse BxPC-3 pancreatic adenocarcinoma cells. Cells were fixed with paraformaldehyde and blocking with 1% BSA and 1% goat serum was performed for 1 hour at 240C. Samples were incubated with primary antibody (1/200: in 1% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody.