製品の概要

  • 製品名Anti-TIPRL antibody
    TIPRL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TIPRL
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 225 and the C-terminus (residue 272) of human TIPRL (NP_690866.1)

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab70795 was affinity purified using an epitope specific to TIPRL immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.
  • 配列類似性Belongs to the TIP41 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1810011K17Rik antibody
    • dJ69E11.3 antibody
    • MGC3794 antibody
    • Putative MAPK activating protein PM10 antibody
    • Putative MAPK-activating protein PM10 antibody
    • RP1-69E11.1 antibody
    • Tip antibody
    • TIP41 antibody
    • TIP41-like protein antibody
    • tiprl antibody
    • TIPRL_HUMAN antibody
    • TOR Signaling Pathway Regulator like antibody
    • Type 2A-interacting protein antibody
    see all

Anti-TIPRL antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling TIPRL with ab70795 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TIPRL antibody (ab70795) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml
    Lane 4 : 293T whole cell lysate at 50 µg/ml
    Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 32 kDa


    Exposure time : 3 seconds
  • Detection of TIPRL by Western Blot of Immunoprecipitate.
    ab70795 at 1µg/ml staining TIPRL in HeLa whole cell lysate immunoprecipitated using ab70795 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-TIPRL antibody (ab70795) 使用論文

ab70795 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70795.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"