Anti-TIPRL 抗体 (ab70795)
Key features and details
- Rabbit polyclonal to TIPRL
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TIPRL antibody -
製品の詳細
Rabbit polyclonal to TIPRL -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 225 and the C-terminus (residue 272) of human TIPRL (NP_690866.1)
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ポジティブ・コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70795 was affinity purified using an epitope specific to TIPRL immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70795の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair. -
配列類似性
Belongs to the TIP41 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 261726 Human
- Entrez Gene: 226591 Mouse
- Omim: 611807 Human
- SwissProt: O75663 Human
- SwissProt: Q8BH58 Mouse
- Unigene: 209431 Human
- Unigene: 21520 Mouse
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別名
- 1810011K17Rik antibody
- dJ69E11.3 antibody
- MGC3794 antibody
see all
画像
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All lanes : Anti-TIPRL antibody (ab70795) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling TIPRL with ab70795 at 1/200 (1µg/ml). Detection: DAB.
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Detection of TIPRL by Western Blot of Immunoprecipitate.
ab70795 at 1µg/ml staining TIPRL in HeLa whole cell lysate immunoprecipitated using ab70795 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab70795 は 5 報の論文で使用されています。
- Xu X et al. Knockdown of TOR signaling pathway regulator suppresses cell migration and invasion in non-small cell lung cancer via the regulation of epithelial-to-mesenchymal transition. Exp Ther Med 19:1925-1932 (2020). PubMed: 32104250
- Luan M et al. TIPRL, a Novel Tumor Suppressor, Suppresses Cell Migration, and Invasion Through Regulating AMPK/mTOR Signaling Pathway in Gastric Cancer. Front Oncol 10:1062 (2020). PubMed: 32719745
- Huang L et al. NUP37 silencing induces inhibition of cell proliferation, G1 phase cell cycle arrest and apoptosis in non-small cell lung cancer cells. Pathol Res Pract 216:152836 (2020). PubMed: 32014308
- Shen H et al. YRDC is upregulated in non-small cell lung cancer and promotes cell proliferation by decreasing cell apoptosis. Oncol Lett 20:43-52 (2020). PubMed: 32565932
- Tirosh O et al. The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions. PLoS Pathog 11:e1005288 (2015). WB . PubMed: 26599541