製品の概要

  • 製品名Anti-TIP120A antibody
    TIP120A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TIP120A
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1180 and 1230 of Human TIP120A (NP_060918.2).

  • ポジティブ・コントロール
    • 293T, NIH3T3 and HeLa cell lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Enhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins.
  • 配列類似性Belongs to the CAND family.
    Contains 27 HEAT repeats.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAND 1 antibody
    • Cand1 antibody
    • CAND1_HUMAN antibody
    • Cullin associated and neddylation dissociated 1 antibody
    • Cullin associated and neddylation dissociated protein 1 antibody
    • Cullin associated NEDD8 dissociated protein 1 antibody
    • Cullin-associated and neddylation-dissociated protein 1 antibody
    • Cullin-associated NEDD8-dissociated protein 1 antibody
    • DKFZP434M1414 antibody
    • FLJ10114 antibody
    • FLJ10929 antibody
    • FLJ38691 antibody
    • FLJ90441 antibody
    • KIAA0829 antibody
    • p120 CAND1 antibody
    • TATA binding protein interacting protein 120A antibody
    • TBP interacting protein antibody
    • TBP interacting protein of 120 kDa A antibody
    • TBP Interacting Protein TIP120A antibody
    • TBP-interacting protein 120A antibody
    • TBP-interacting protein of 120 kDa A antibody
    • TIP120 antibody
    • TIP120 protein antibody
    see all

Anti-TIP120A antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse hybridoma tumor (right) tissues labelling TIP120A with ab101987 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TIP120A antibody (ab101987) at 0.1 µg

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg


    Predicted band size : 136 kDa
  • Detection of TIP120A by Western blot of Immunprecipitate.
    ab101987, at 1 µg/ml, staining TIP120A in HeLa whole cell lysate immunoprecipitated using ab101987 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-TIP120A antibody (ab101987) 使用論文

ab101987 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101987.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"