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RabMAb

Anti-TIMP1 抗体 [EP1549RY] (ab81282)

製品の概要

  • 製品名
    Anti-TIMP1 antibody [EP1549RY]
    TIMP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1549RY] to TIMP1
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C-terminal region of human TIMP1 was used as immunogen.

  • ポジティブ・コントロール
    • recombinant protein
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 23 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能
      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
    • 配列類似性
      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • 翻訳後修飾
      The activity of TIMP1 is dependent on the presence of disulfide bonds.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Clgi antibody
      • Collagenase inhibitor antibody
      • Collagenase inhibitor, Human antibody
      • EPA antibody
      • EPO antibody
      • Erythroid Potentiating Activity antibody
      • Erythroid-potentiating activity antibody
      • Fibroblast collagenase inhibitor antibody
      • FLJ90373 antibody
      • HCI antibody
      • Human Collagenase Inhibitor antibody
      • Metalloproteinase inhibitor 1 antibody
      • Metalloproteinase inhibitor 1 precursor antibody
      • OTTHUMP00000023214 antibody
      • TIMP 1 antibody
      • TIMP antibody
      • TIMP metallopeptidase inhibitor 1 antibody
      • TIMP-1 antibody
      • Timp1 antibody
      • TIMP1 protein antibody
      • TIMP1_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 1 antibody
      • Tissue inhibitor of metalloproteinases 1 antibody
      • Tissue inhibitor of metalloproteinases antibody
      • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
      see all

    画像

    • Anti-TIMP1 antibody [EP1549RY] (ab81282) at 1/500 dilution + 5 ng of recombinant protein

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab81282 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Dog Tissue sections (Left Ventricle)
    Specification
    Left Ventricle
    Permeabilization
    Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
    Fixative
    Paraformaldehyde
    Username

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    投稿 Aug 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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