Anti-TIMP1 抗体 - Carboxyterminal end (ab38978)
Key features and details
- Rabbit polyclonal to TIMP1 - Carboxyterminal end
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TIMP1 antibody - Carboxyterminal end
TIMP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TIMP1 - Carboxyterminal end -
由来種
Rabbit -
特異性
This antibody recognizes both reduced and non reduced TIMP1 protein, but does not cross react with other TIMP family members (TIMP2, TIMP3, TIMP4). -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide based on the carboxyterminal region of human TIMP1.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38978の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/1000 - 1/5000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa). Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 29 kDa when used against the reduced protein. Higher concentration of antibody may be needed for non human samples. Dilution optimised using Chromogenic detection.
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml.
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab28261 as Capture. |
特記事項 |
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WB
1/1000 - 1/5000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa). Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 29 kDa when used against the reduced protein. Higher concentration of antibody may be needed for non human samples. Dilution optimised using Chromogenic detection. |
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab28261 as Capture. |
ターゲット情報
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻訳後修飾
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
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別名
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
see all
画像
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All lanes : Anti-TIMP1 antibody - Carboxyterminal end (ab38978) at 1/1000 dilution
Lane 1 : Human TIMP1
Lane 2 : Cell media from human chondrosarcoma (no treatment)
Lane 3 : Cell media from human chondrosarcoma (treated with TPA)
Predicted band size: 23 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted? -
Each lane corresponds to a distinct lung adenocarcinoma patient sample. The primary antibody was used at 1/3000 dilution. 10µg/ml lysate has been added per lane. A sheep anti-rabbit monoclonal conjugated to HRP was used as the secondary antibody at 1/10,000 dilution.
Detection method used was Western Lightning. 3 minutes. Performed under reducing conditions.
Samples blocked using 5% BSA for 1 hour at 25°C.
Non-specific band at 27kDa is thought to be Ig light chain.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (34)
ab38978 は 34 報の論文で使用されています。
- Chalise U et al. Harnessing the Plasma Proteome to Mirror Current and Predict Future Cardiac Remodeling After Myocardial Infarction. J Cardiovasc Transl Res 16:3-16 (2023). PubMed: 36197585
- Qiao P et al. Dapagliflozin protects against nonalcoholic steatohepatitis in db/db mice. Front Pharmacol 13:934136 (2022). PubMed: 36059948
- Han H et al. Oncostatin M promotes infarct repair and improves cardiac function after myocardial infarction. Am J Transl Res 13:11329-11340 (2021). PubMed: 34786061
- Chen Y et al. The circRNA-MYLK plays oncogenic roles in the Hep-2 cell line by sponging microRNA-145-5p. Gen Physiol Biophys 39:229-237 (2020). PubMed: 32525816
- Chen X et al. Circular RNA circFBXW4 suppresses hepatic fibrosis via targeting the miR-18b-3p/FBXW7 axis. Theranostics 10:4851-4870 (2020). PubMed: 32308754