製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TIMP1
  • アプリケーション
    適用あり: WB, Neutralising, Indirect ELISA, Sandwich ELISA, Blockingmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant human TIMP1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 23 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TIMP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 5 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Human TIMP1 (3.0 µg/ml). sELISA: Use at a concentration of 0.5 - 2 µg/ml (using 100µl/well antibody solution).
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100µl/well antibody solution).
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TIMP1 (Biotin) (ab77848). Can be used as capture antibody with recommended pair. Use 100µl/well antibody solution.
Blocking Use at an assay dependent concentration. PubMed: 22016393

ターゲット情報

  • 機能
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
  • 配列類似性
    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • 翻訳後修飾
    The activity of TIMP1 is dependent on the presence of disulfide bonds.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Clgi antibody
    • Collagenase inhibitor antibody
    • Collagenase inhibitor, Human antibody
    • EPA antibody
    • EPO antibody
    • Erythroid Potentiating Activity antibody
    • Erythroid-potentiating activity antibody
    • Fibroblast collagenase inhibitor antibody
    • FLJ90373 antibody
    • HCI antibody
    • Human Collagenase Inhibitor antibody
    • Metalloproteinase inhibitor 1 antibody
    • Metalloproteinase inhibitor 1 precursor antibody
    • OTTHUMP00000023214 antibody
    • TIMP 1 antibody
    • TIMP antibody
    • TIMP metallopeptidase inhibitor 1 antibody
    • TIMP-1 antibody
    • Timp1 antibody
    • TIMP1 protein antibody
    • TIMP1_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 1 antibody
    • Tissue inhibitor of metalloproteinases 1 antibody
    • Tissue inhibitor of metalloproteinases antibody
    • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
    see all

画像

  • ab77847 was used to block TIMP1 in MDA-MB-231-BM cells.
    Control = HAS2-expressing cells
    C2-4 and C4-25 = HAS2 knockdown cells

プロトコール

参考文献

This product has been referenced in:
  • Forte D  et al. The tissue inhibitor of metalloproteinases-1 (TIMP-1) promotes survival and migration of acute myeloid leukemia cells through CD63/PI3K/Akt/p21 signaling. Oncotarget 8:2261-2274 (2017). Flow Cyt . Read more (PubMed: 27903985) »
  • Bernert B  et al. Hyaluronan Synthase 2 (HAS2) Promotes Breast Cancer Cell Invasion by Suppression of Tissue Metalloproteinase Inhibitor 1 (TIMP-1). J Biol Chem 286:42349-59 (2011). Blocking . Read more (PubMed: 22016393) »

See all 2 Publications for this product

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