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Our Abpromise guarantee covers the use of ab2464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling TIMP1 with undiluted ab2464. Tissue sections were fixed in formaldehyde; heat-mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C, followed by incubation with Anti-TIMP1 antibody [2A5] (ab2464) for 12 hours at 20 °C in PBS. A biotin conjugate goat anti-mouse secondary antibody was used at 1/100 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"