製品の概要

  • 製品名Anti-TIMM50 antibody [EPR5784]
    TIMM50 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5784] to TIMM50
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a region within Human TIMM50.

  • ポジティブ・コントロール
    • 293T, SH SY5Y, HepG2 and HeLa cell lysates Human kidney tissue, human liver tissue (IHC-P)
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
    • 組織特異性Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
    • 配列類似性Belongs to the TIM50 family.
      Contains 1 FCP1 homology domain.
    • ドメインThe FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
    • 細胞内局在Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Homolog of yeast Tim50 antibody
      • Import inner membrane translocase subunit TIM 50 antibody
      • Import inner membrane translocase subunit TIM50 antibody
      • Import inner membrane translocase subunit TIM50 mitochondrial antibody
      • MGC102733 antibody
      • Mitochondrial import inner membrane translocase subunit TIM50 antibody
      • TIM 50 antibody
      • TIM 50L antibody
      • TIM50 antibody
      • Tim50 like protein antibody
      • TIM50_HUMAN antibody
      • TIM50L antibody
      • TIMM 50 antibody
      • TIMM50 antibody
      • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
      • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
      • Translocase of inner mitochondrial membrane 50 homolog antibody
      • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
      see all

    Anti-TIMM50 antibody [EPR5784] 画像

    • All lanes : Anti-TIMM50 antibody [EPR5784] (ab109527) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 40 kDa
    • ab109527, at 1/50, staining TIMM50 in paraffin-embedded kidney tissue by Immunohistochemistry.
    • ab109527, at 1/50, staining TIMM50 in paraffin-embedded liver tissue by Immunohistochemistry.

    Anti-TIMM50 antibody [EPR5784] (ab109527) 使用論文

    ab109527 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab109527.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"