製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/16000.
WB Use a concentration of 0.5 - 3 µg/ml. Detects a band of approximately 40 kDa.

ターゲット情報

  • 機能Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
  • 組織特異性Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
  • 配列類似性Belongs to the TIM50 family.
    Contains 1 FCP1 homology domain.
  • ドメインThe FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
  • 細胞内局在Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homolog of yeast Tim50 antibody
    • Import inner membrane translocase subunit TIM 50 antibody
    • Import inner membrane translocase subunit TIM50 antibody
    • Import inner membrane translocase subunit TIM50 mitochondrial antibody
    • MGC102733 antibody
    • Mitochondrial import inner membrane translocase subunit TIM50 antibody
    • TIM 50 antibody
    • TIM 50L antibody
    • TIM50 antibody
    • Tim50 like protein antibody
    • TIM50_HUMAN antibody
    • TIM50L antibody
    • TIMM 50 antibody
    • TIMM50 antibody
    • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
    • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
    • Translocase of inner mitochondrial membrane 50 homolog antibody
    • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
    see all

Anti-TIMM50 antibody 画像

  • Anti-TIMM50 antibody (ab23938) at 0.5 µg/ml

    Performed under reducing conditions.

Anti-TIMM50 antibody (ab23938) 使用論文

This product has been referenced in:
  • Byun J  et al. CR6-interacting factor 1 is a key regulator in Aß-induced mitochondrial disruption and pathogenesis of Alzheimer's disease. Cell Death Differ 22:959-73 (2015). WB ; Mouse . Read more (PubMed: 25361083) »
  • Perciavalle RM  et al. Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol 14:575-83 (2012). Read more (PubMed: 22544066) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100/PBS
Blocking step 0.5% BSA, 0.2% fish skin gelatin/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
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投稿 May 28 2010

Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount 25 µg
Specification meiotic egg extract
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH/3T3 Fibroblasts)
Loading amount 20 µg
Specification NIH/3T3 Fibroblasts
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa post nuclear cell lysate)
Loading amount 30 µg
Specification HeLa post nuclear cell lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
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投稿 Aug 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"