製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to TIMM13
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human) with tag

  • ポジティブ・コントロール
    • Recombinant tagged TIMM13.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    WB: 1/1000 - 1/2500. Predicted molecular weight: 10 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins.
    • 組織特異性
      Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
    • 配列類似性
      Belongs to the small Tim family.
    • ドメイン
      The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.
    • 細胞内局在
      Mitochondrion inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Mitochondrial import inner membrane translocase subunit TIM13 antibody
      • Mitochondrial import inner membrane translocase subunit Tim13B antibody
      • ppv1 antibody
      • TIM 13 antibody
      • TIM13 antibody
      • TIM13_HUMAN antibody
      • TIM13B antibody
      • TIMM 13 antibody
      • Timm13 antibody
      • TIMM13A antibody
      • TIMM13B antibody
      • Translocase of inner mitochondrial membrane 13 homolog antibody
      see all

    画像



    • Predicted band size : 10 kDa

    プロトコール

    参考文献

    ab66142 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Drosophila melanogaster Tissue lysate - whole (whole body)
    Gel Running Conditions
    Reduced Denaturing (4-20% Bis/Tris)
    Loading amount
    50 µg
    Specification
    whole body
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 06 2017

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - other (HEK293)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Other - Protein G dynabeads
    Specification
    HEK293
    Username

    Dr. Edyta Marcon

    Verified customer

    投稿 Jun 26 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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