製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TIMM13
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Horse, Chicken, Guinea pig, Cow, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N-terminal amino acids 5-54 (FGSDFGGTGG GKLDPGAIME QVKVQIAVAN AQELLQRMTD KCFRKCIGKP)of Mouse TIMM13 (NP_038923).

  • ポジティブ・コントロール
    • Mouse Kidney Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins.
  • 組織特異性
    Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
  • 配列類似性
    Belongs to the small Tim family.
  • ドメイン
    The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Mitochondrial import inner membrane translocase subunit TIM13 antibody
    • Mitochondrial import inner membrane translocase subunit Tim13B antibody
    • ppv1 antibody
    • TIM 13 antibody
    • TIM13 antibody
    • TIM13_HUMAN antibody
    • TIM13B antibody
    • TIMM 13 antibody
    • Timm13 antibody
    • TIMM13A antibody
    • TIMM13B antibody
    • Translocase of inner mitochondrial membrane 13 homolog antibody
    see all

画像

  • Anti-TIMM13 antibody (ab113781) at 1 µg/ml + Mouse Kidney Lysate at 10 µg

    Predicted band size : 11 kDa

プロトコール

参考文献

ab113781 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart (Embryonic))
Permeabilization
Yes - Tween-20
Specification
Heart (Embryonic)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Aug 29 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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