製品の概要

  • 製品名Anti-Timeless antibody [EPR5275]
    Timeless 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5275] to Timeless
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human Timeless.

  • ポジティブ・コントロール
    • HeLa, Jurkat, JAR, and BxPC-3 cell lysates, Human colon and Human tonsil tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/200000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
    • 組織特異性Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis.
    • 配列類似性Belongs to the timeless family.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ12640 antibody
      • FLJ20714 antibody
      • hTIM antibody
      • Protein timeless homolog antibody
      • TIM antibody
      • TIM_HUMAN antibody
      • TIM1 antibody
      • Timeless antibody
      • timeless circadian clock 1 antibody
      • timeless circadian clock antibody
      • timeless homolog antibody
      • TIMELESS1 antibody
      • Tof1 homolog antibody
      see all

    Anti-Timeless antibody [EPR5275] 画像

    • All lanes : Anti-Timeless antibody [EPR5275] (ab109512) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 139 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109512 at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109512 at a dilution of 1/100.

    Anti-Timeless antibody [EPR5275] (ab109512) 使用論文

    ab109512 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109512.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"