Anti-Timeless 抗体 (ab50943)

製品の概要

  • 製品名Anti-Timeless antibody
    Timeless 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Timeless
  • 特異性This antibody reacts with Timeless
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Cow, Dog, Drosophila melanogaster
  • 免疫原

    Synthetic peptide: MDLHMMNCEL LATCSALGYL EGDTYHKEPD CLESVKDLIR YLRHEDETRD, corresponding to amino acids 1-50 of Human Timeless

  • ポジティブ・コントロール
    • Transfected 293T cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab50943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).Can be blocked with Timeless peptide (ab113078). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
  • 組織特異性Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis.
  • 配列類似性Belongs to the timeless family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ12640 antibody
    • FLJ20714 antibody
    • hTIM antibody
    • Protein timeless homolog antibody
    • TIM antibody
    • TIM_HUMAN antibody
    • TIM1 antibody
    • Timeless antibody
    • timeless circadian clock 1 antibody
    • timeless circadian clock antibody
    • timeless homolog antibody
    • TIMELESS1 antibody
    • Tof1 homolog antibody
    see all

Anti-Timeless antibody 画像

  • All lanes : Anti-Timeless antibody (ab50943) at 1/500 dilution

    Lane 1 : Whole cell extract from HeLa cells at 100 µg
    Lane 2 : Whole cell extract from HeLa cells at 200 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 139 kDa


    Exposure time : 50 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Anti-Timeless antibody (ab50943) at 2.5 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 139 kDa
    Observed band size : 139 kDa

Anti-Timeless antibody (ab50943) 使用論文

ab50943 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"