Anti-TIF1 gamma 抗体 (ab47062)

Lanes 1 - 4: Merged signal (red and green). Green - ab47062 observed at 150 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab47062 was shown to recognize TIF1 gamma in wild-type HAP1 cells as signal was lost at the expected MW in TRIM33 (TIF1 gamma) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TRIM33 (TIF1 gamma) knockout samples were subjected to SDS-PAGE. Ab47062 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

ab47062 stained in MCF7 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab47062 at 5 µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.