製品の概要
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製品名Anti-TIF1 gamma antibody
TIF1 gamma 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TIF1 gamma
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アプリケーション適用あり: WB, ICC/IFmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Macaque monkey
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human TIF1 gamma.
(Peptide available as ab47108.)
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ポジティブ･コントロール
- ab47062 gave a positive signal in the following whole cell lysates: F9 (Mouse embryonic carcinoma cell line); E14tG2a (Mouse embryonic stem cell line); HeLa (Human epithelial carcinoma cell line) (data not shown); MEF1 (Mouse embryonic fibroblast cell line) (data not shown); MEL-1 (Human embryonic stem cell, male cell line) (data not shown); Mouse Embryonic Germ cell (data not shown). ICC-IF: mouse embryonic stem cells and MCF7 cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab47062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 122 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
ターゲット情報
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機能Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
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組織特異性Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.
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パスウェイProtein modification; protein ubiquitination.
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関連疾患Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM33 is found in thyroid papillary carcinomas. Translocation t(1;10)(p13;q11) with RET. The translocation generates the TRIM33/RET (PTC7) oncogene.
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配列類似性Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
細胞内局在Nucleus. In discrete nuclear dots resembling nuclear bodies.
- Information by UniProt
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参照データベース
- Entrez Gene: 51592 Human
- Entrez Gene: 94093 Mouse
- Omim: 605769 Human
- SwissProt: Q9UPN9 Human
- SwissProt: Q99PP7 Mouse
- Unigene: 26837 Human
- Unigene: 195036 Mouse
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別名
- 8030451N04Rik antibody
- AI413936 antibody
- cb1085 antibody
see all
Anti-TIF1 gamma antibody 画像
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Western blot - TIF1 gamma antibody (ab47062)All lanes : Anti-TIF1 gamma antibody (ab47062) at 1 µg/ml
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
Lane 2 : E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 122 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : 220 kDa. We are unsure as to the identity of these extra bands.The 150 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to TIF1 gamma. -
Immunocytochemistry/ Immunofluorescence - Anti-TIF1 gamma antibody (ab47062)ab47062 stained in MCF7 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab47062 at 5 µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Immunocytochemistry/ Immunofluorescence - TIF1 gamma antibody (ab47062)ICC/IF image of ab47062 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47062, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). -
Western blot - Anti-TIF1 gamma antibody (ab47062)All lanes : Anti-TIF1 gamma antibody (ab47062) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 122 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : 220 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Anti-TIF1 gamma antibody (ab47062) 使用論文
ab47062 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"