Anti-TIF1 alpha 抗体 (ab70560)

製品の概要

  • 製品名Anti-TIF1 alpha antibody
    TIF1 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TIF1 alpha
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 700 and 750 of human TIF1 alpha (NP_056989.2)

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human colon adenocarcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
  • パスウェイProtein modification; protein ubiquitination.
  • 関連疾患Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
  • 配列類似性Contains 2 B box-type zinc fingers.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated.
  • 細胞内局在Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin protein ligase TRIM24 antibody
    • E3 ubiquitin-protein ligase Trim24 antibody
    • hTIF1 antibody
    • PTC6 antibody
    • RING finger protein 82 antibody
    • RNF82 antibody
    • TF1A antibody
    • TIF1 antibody
    • TIF1-alpha antibody
    • TIF1A antibody
    • TIF1A_HUMAN antibody
    • TIF1ALPHA antibody
    • Transcription intermediary factor 1-alpha antibody
    • Transcriptional intermediary factor 1 alpha antibody
    • Transcriptional intermediary factor 1 antibody
    • Trim24 antibody
    • Tripartite motif containing 24 antibody
    • Tripartite motif-containing protein 24 antibody
    see all

Anti-TIF1 alpha antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TIF1 alpha with ab70560 at 1/1000 (0.2µg/ml). Detection: DAB.
  • IHC image of TIF1 alpha staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70560, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TIF1 alpha antibody (ab70560) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3whole cell lysate at 50 µg


    Predicted band size : 116 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

Anti-TIF1 alpha antibody (ab70560) 使用論文

ab70560 has not yet been referenced specifically in any publications.

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Thank you for contacting us.
We have 3 antibodies to TIF1 alpha: ab70560, ab57994 and ab84381.
Two of them have been tested in mouse (ab70560 and ab84381), and ab84381 was already tested and would be guaranteed for mouse and human paraffin-em...

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Specification Brain
Blocking step No blocking step used for 30 minute(s) · Concentration: 4%
Fixative Formaldehyde
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投稿 Aug 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"