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Our Abpromise guarantee covers the use of ab24859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/25. PubMed: 19253934|
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 126 kDa.|
|ELISA||Use a concentration of 1 - 2 µg/ml.|
|Flow Cyt||Use 1-2µg for 106 cells.
We tested in-house with methanol-fixed cells, but this may not be necessary since the antibody recognies an extracellular epitope.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration. PubMed: 20622121|
ab24859 staining TIE2 in C57 Mouse melanoma tumour tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Tween 20 and blocked with 3% BSA for 1 hour at 23°C; antigen retrieval was by heat mediation in a Tris/HCI buffer. Samples were incubated with primary antibody (1/25 in 1% BSA, 0.5% Tween 20 and 1% Donkey serum) for 9 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey polyclonal (1/1000) was used as the secondary antibody.
ab24859 staining TIE2 in Human arterial wall tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 3% serum for 30 minutes at 25°C. Tissues were dunked in formalin for 72 hours before being embedded in OCT and sectioned. The sample was incubated with primary antibody (1/100 in 3% horse serum) at 4°C for 15 hours. A HRP-conjugated Horse vector immpress was used as the secondary antibody.