製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TIE1
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to C terminus of human TIE1

  • ポジティブ・コントロール
    • Human tonsil tissue
  • 特記事項
    Cell membrane staining pattern

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/100 for 30 min at room temperature.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
    • 組織特異性
      Specifically expressed in developing vascular endothelial cells.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
      Contains 3 EGF-like domains.
      Contains 3 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • JKT 14 antibody
      • JTK14 antibody
      • TIE antibody
      • Tie1 antibody
      • TIE1_HUMAN antibody
      • Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody
      • Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody
      • Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody
      • Tyrosine protein kinase receptor Tie 1 antibody
      • Tyrosine-protein kinase receptor Tie-1 antibody
      see all

    画像

    • ab64477, at a dilution of 1/100, staining TIE1 in formalin fixed, paraffin embedded human tonsil tissue sections by Immunohistochemistry.

    プロトコール

    参考文献

    ab64477 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Uterus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.0, 96-99¯C, 20 min.
    Permeabilization
    No
    Specification
    Uterus
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 20°C
    Fixative
    Formalin
    Username

    Abcam user community

    Verified customer

    投稿 Dec 12 2016

    Thank you for your enquiry. Unfortunately, I am sorry we are not able to release the immunogen sequence for this particular antibody as it is considered commercially sensitive. However, our source for this antibody has confirmed that the sequen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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