製品の概要

  • 製品名Anti-TICAM2 antibody [mAbcam77169]
    TICAM2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam77169] to TICAM2
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human TICAM2.

    (Peptide available as ab57980.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Kidney and Human Spleen Tissue Lyates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名mAbcam77169
  • ミエローマSp2/0-Ag14
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Functions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1.
  • 組織特異性Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas.
  • 配列類似性Contains 1 TIR domain.
  • ドメインThe TIR domain mediates the interaction with TRAF6.
  • 翻訳後修飾Phosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
    Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.
  • 細胞内局在Cytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Putative NF-kappa-B-activating protein 502 antibody
    • TCAM2_HUMAN antibody
    • TICAM-2 antibody
    • TICAM2 antibody
    • TIR containing adapter molecule 2 antibody
    • TIR domain containing adaptor protein antibody
    • TIR domain-containing adapter molecule 2 antibody
    • TIRAP 3 antibody
    • TIRAP3 antibody
    • TIRAP3a short form antibody
    • TIRP antibody
    • Toll-like receptor adaptor protein 3 antibody
    • Toll/interleukin-1 receptor domain-containing protein antibody
    • TRAM antibody
    • TRIF-related adapter molecule antibody
    see all

Anti-TICAM2 antibody [mAbcam77169] 画像

  • All lanes : Anti-TICAM2 antibody [mAbcam77169] (ab77169) at 10 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing PC3 cells stained with ab77169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77169, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-TICAM2 antibody [mAbcam77169] (ab77169) 使用論文

ab77169 has not yet been referenced specifically in any publications.

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