製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TIAM2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TIAM2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse hippocampus; Mouse cerebellum; Rat cortex; Rat olfactory bulb.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 210 kDa (predicted molecular weight: 193 kDa).

ターゲット情報

  • 機能
    Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA.
  • 組織特異性
    Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis.
  • 配列類似性
    Belongs to the TIAM family.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 RBD (Ras-binding) domain.
  • ドメイン
    The PH 1 domain and amino acids 621-782 (a region called TSS; otherwise known as CC-Ex) are necessary for membrane localization. The PH 1 and TSS domains are necessary for Rac1 activity. The PH 2 domain is engaged in the enhancement of the catalytic activity of the adjacent DH domain. The PH 1, TSS and DH domains are necessary to induce neurite-like structure.
  • 翻訳後修飾
    Phosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth.
  • 細胞内局在
    Cytoplasm. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Localizes to the plasma membrane in neurites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ41865 antibody
    • OTTHUMP00000017472 antibody
    • SIF and TIAM1 like exchange factor antibody
    • SIF and TIAM1-like exchange factor antibody
    • STEF antibody
    • T cell lymphoma invasion and metastasis 2 antibody
    • T lymphoma invasion and metastasis inducing protein 2 antibody
    • T-lymphoma invasion and metastasis-inducing protein 2 antibody
    • TIAM 2 antibody
    • TIAM-2 antibody
    • Tiam2 antibody
    • TIAM2_HUMAN antibody
    see all

画像

  • All lanes : Anti-TIAM2 antibody (ab93839) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Hippocampus Tissue Lysate
    Lane 3 : Rat Cortex Tissue Lysate
    Lane 4 : Cerebellum Mouse Tissue Lysate
    Lane 5 : Rat Olfactory Bulb Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 193 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

プロトコール

参考文献

ab93839 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (3-8%)
Sample
Human Cell lysate - whole cell (Hek293, U2OS, HepG2)
Specification
Hek293, U2OS, HepG2
Blocking step
Caesin blocking buffer (Sigma) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Aishwarya Payapilly

Verified customer

投稿 Apr 10 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Human Cell (U2OS)
Specification
U2OS
Permeabilization
Yes - 0.1% Triton-X-100
Fixative
Formaldehyde
Username

Dr. Aishwarya Payapilly

Verified customer

投稿 Apr 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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