製品の概要

  • 製品名Anti-TIAM2 antibody
    TIAM2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to TIAM2
  • 特異性ab123810 is expected to recognize isoform a (NP_036586.2) only.
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog, Pig
  • 免疫原

    Synthetic peptide:

    TLQGSKNHSNT-C

    , corresponding to N terminal amino acids 9-19 of Human TIAM2 according to NP_036586.2.

  • 特記事項Preliminary experiments gave bands at approx 150kDa and 37kDa in Mouse and Rat Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 192kDa according to mouse NP_036008.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA.
  • 組織特異性Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis.
  • 配列類似性Belongs to the TIAM family.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 RBD (Ras-binding) domain.
  • ドメインThe PH 1 domain and amino acids 621-782 (a region called TSS; otherwise known as CC-Ex) are necessary for membrane localization. The PH 1 and TSS domains are necessary for Rac1 activity. The PH 2 domain is engaged in the enhancement of the catalytic activity of the adjacent DH domain. The PH 1, TSS and DH domains are necessary to induce neurite-like structure.
  • 翻訳後修飾Phosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth.
  • 細胞内局在Cytoplasm. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Localizes to the plasma membrane in neurites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ41865 antibody
    • OTTHUMP00000017472 antibody
    • SIF and TIAM1 like exchange factor antibody
    • SIF and TIAM1-like exchange factor antibody
    • STEF antibody
    • T cell lymphoma invasion and metastasis 2 antibody
    • T lymphoma invasion and metastasis inducing protein 2 antibody
    • T-lymphoma invasion and metastasis-inducing protein 2 antibody
    • TIAM 2 antibody
    • TIAM-2 antibody
    • Tiam2 antibody
    • TIAM2_HUMAN antibody
    see all

Anti-TIAM2 antibody 画像

  • ab123810 (5µg/ml) staining of paraffin embedded Human Testis following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoskeletal staining in interstitial (Leydig) cells.

Anti-TIAM2 antibody (ab123810) 使用論文

ab123810 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"