製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TIAM2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human TIAM2 Isoform 3 (NP_001010927.1, amino acids 1-626).

  • ポジティブ・コントロール
    • K562 cell lysate; TIAM2 transfected 293T cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: Isoform 3, 71 kDa.

ターゲット情報

  • 機能
    Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA.
  • 組織特異性
    Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis.
  • 配列類似性
    Belongs to the TIAM family.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 RBD (Ras-binding) domain.
  • ドメイン
    The PH 1 domain and amino acids 621-782 (a region called TSS; otherwise known as CC-Ex) are necessary for membrane localization. The PH 1 and TSS domains are necessary for Rac1 activity. The PH 2 domain is engaged in the enhancement of the catalytic activity of the adjacent DH domain. The PH 1, TSS and DH domains are necessary to induce neurite-like structure.
  • 翻訳後修飾
    Phosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth.
  • 細胞内局在
    Cytoplasm. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Localizes to the plasma membrane in neurites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ41865 antibody
    • OTTHUMP00000017472 antibody
    • SIF and TIAM1 like exchange factor antibody
    • SIF and TIAM1-like exchange factor antibody
    • STEF antibody
    • T cell lymphoma invasion and metastasis 2 antibody
    • T lymphoma invasion and metastasis inducing protein 2 antibody
    • T-lymphoma invasion and metastasis-inducing protein 2 antibody
    • TIAM 2 antibody
    • TIAM-2 antibody
    • Tiam2 antibody
    • TIAM2_HUMAN antibody
    see all

画像

  • Anti-TIAM2 antibody (ab103578) at 5 µg/ml + K562 cell lysate at 50 µg

    Predicted band size : Isoform 3, 71 kDa
  • All lanes : Anti-TIAM2 antibody (ab103578) at 5 µg/ml

    Lane 1 : TIAM2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : Isoform 3, 71 kDa

プロトコール

参考文献

ab103578 has not yet been referenced specifically in any publications.

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