Anti-Thyroid Peroxidase 抗体 [MoAb47] (ab12500)

製品の概要

  • 製品名Anti-Thyroid Peroxidase antibody [MoAb47]
    Thyroid Peroxidase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MoAb47] to Thyroid Peroxidase
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human Thyroid Peroxidase purified from thyroid microsomes by immunoaffinity chromatography

  • ポジティブ・コントロール
    • Thyroid

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報IHC-P: 1/10 - 1/25 in an ABC method. Perform heat mediated antigen retrieval with 1mM EDTA buffer, pH 9.0 before commencing with IHC staining protocol. We suggest an incubation period of 30-60 minutes at room temperature.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
    • パスウェイHormone biosynthesis; thyroid hormone biosynthesis.
    • 関連疾患Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
      Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
    • 配列類似性Belongs to the peroxidase family. XPO subfamily.
      Contains 1 EGF-like domain.
      Contains 1 Sushi (CCP/SCR) domain.
    • 翻訳後修飾Glycosylated.
      Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
      Cleaved in its N-terminal part.
    • 細胞内局在Membrane and Cell surface.
    • Information by UniProt
    • 参照データベース
    • 別名
      • MSA antibody
      • PERT_HUMAN antibody
      • TDH2A antibody
      • Thyroid microsomal antigen antibody
      • Thyroid peroxidase antibody
      • Thyroperoxidase antibody
      • TPO antibody
      • TPX antibody
      see all

    Anti-Thyroid Peroxidase antibody [MoAb47] (ab12500) 使用論文

    ab12500 has not yet been referenced specifically in any publications.

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    Thank you for your enquiry.

    I can confirm that ab12500 Anti-Thyroid Peroxidase antibody [MoAb47]antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernata...

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    Thank you for contacting us.

    According to the datasheet, this antibody is a non-purified cell culture supernatant product. That means, along with whole serum andascitis products, the antibody concentration cannot be determined as there are t...

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    Thank you for providing some further information. I understand your customer has used up most of the product during testing and optimization. This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vi...

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    Thank you for getting back to me and for providing some further details. I understand that whilst ab818 failed to work In Western blot and in Dot blot; the other antibody ab12500 seemed to be fine. It is important to mention that these antibodies detec...

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    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"