ab63962 is expected to recognize both reported isoforms of Thyroid Hormone Receptor alpha (THRA) (NP_955366.1; NP_003241.2). This antibody is not expected to cross-react to Thyroid Hormone Receptor beta (THRB).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution 1:32/000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects bands of approximately 60 and 50 kDa (predicted molecular weights: 54 and 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TR alpha and TR beta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3 triiodo L thyronine (T3). Thyroid hormones, through their interaction with the TR affect metabolic processes, growth and development.