Anti-Thyroid Hormone Receptor alpha 1+2 抗体 (ab42565)

製品の概要

  • 製品名
    Anti-Thyroid Hormone Receptor alpha 1+2 antibody
    Thyroid Hormone Receptor alpha 1+2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
  • アプリケーション
    適用あり: WB, IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig, Zebrafish
  • 免疫原

    A peptide corresponding to a region of Human THRA

  • ポジティブ・コントロール
    • Jurkat cell lysate and human skin tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 58, 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

ICC/IF 1/100.

ターゲット情報

画像

  • Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58, 47 kDa
    Observed band size : 47,58 kDa
  • ab42565 at 4ug/ml concentration, staining human THRA in squamous epithelial cells of human skin (indicated by arrows) by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

参考文献

ab42565 has not yet been referenced specifically in any publications.

レビューと Q&A



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Expiration date: November 16th, 2012

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human TRa expressed in CHO-K1)
Specification
Human TRa expressed in CHO-K1
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

投稿 Aug 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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