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Our Abpromise guarantee covers the use of ab14335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.
Tested in lung and heart tissues.
ICC/IF image of ab14335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14335, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14335 at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hear tissue sections labeling Thymosin beta 4 with ab14335 at 1/100 dilution, followed by an appropriate secondary antibody conjugated to Cy3. The nuclei were stained with DAPI.
Indirect ELISA analysis. Anti-Thymosin beta 4 antibody (ab14335) at 1/1000 dilution. Sample: chicken heart culture serum. An alkaline phosphatase conjugated donkey anti-rabbit secondary antibody was used at 1/8000
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