製品の概要

  • 製品名Anti-Thymosin beta 10 antibody
    Thymosin beta 10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Thymosin beta 10
  • 特異性Human Thymosin B10 (aa 38-43). No cross reactivities with human Thymosin B4, Thymosin B9, Thymosin B15, Thymosin B4 (aa 1-4) peptide, or Thymosin B4 (aa 1-14).
  • アプリケーション適用あり: ELISA, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide:

    KRSEIS

    conjugated to KLH, corresponding to amino acids 38-43 of Human Thymosin beta 10.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/4.
WB Use at an assay dependent concentration. Predicted molecular weight: 5 kDa. PubMed: 18854659
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

Anti-Thymosin beta 10 antibody 画像

  • ICC/IF image of ab14338 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14338, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14338 at 1/1000 dilution.

Anti-Thymosin beta 10 antibody (ab14338) 使用論文

This product has been referenced in:
  • Lagarrigue M  et al. Revisiting Rat Spermatogenesis with MALDI Imaging at 20-{micro}m Resolution. Mol Cell Proteomics 10:M110.005991 (2011). IHC-P ; Rat . Read more (PubMed: 21149303) »
  • Zhang T  et al. Overexpression of thymosin beta-10 inhibits VEGF mRNA expression, autocrine VEGF protein production, and tube formation in hypoxia-induced monkey choroid-retinal endothelial cells. Ophthalmic Res 41:36-43 (2009). WB ; Human . Read more (PubMed: 18854659) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast)
Specification breast
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
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投稿 Jan 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"