Anti-Thymine DNA glycosylase 抗体 (ab106301)

製品の概要

  • 製品名
    Anti-Thymine DNA glycosylase antibody
    Thymine DNA glycosylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Thymine DNA glycosylase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Chicken, Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Thymine DNA glycosylase .

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; A431; Hues7.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
  • 配列類似性
    Belongs to the TDG/mug DNA glycosylase family.
  • 翻訳後修飾
    Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C JUN leucine zipper interactive protein antibody
    • C JUN leucine zipper interactive protein antibody
    • C-JUN leucine zipper interactive protein JZA-3 antibody
    • E130317C12Rik antibody
    • EC 3.2.2.29 antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch-specific thymine DNA glycosylase antibody
    • JZA 3 antibody
    • Jza1 antibody
    • T:G mismatch thymine glycosylase antibody
    • Tdg antibody
    • TDG_HUMAN antibody
    • Thymine DNA glycosylase antibody
    • Thymine-DNA glycosylase antibody
    see all

画像

  • Thymine DNA glycosylase was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Thymine DNA glycosylase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab106301.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 50kDa; Thymine DNA glycosylase

  • All lanes : Anti-Thymine DNA glycosylase antibody (ab106301) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 22 kDa, 78 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes
  • All lanes : Anti-Thymine DNA glycosylase antibody (ab106301) at 1 µg/ml

    All lanes : Mouse ES whole cell lysate

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds

    See Abreview

参考文献

ab106301 has not yet been referenced specifically in any publications.

レビューと Q&A

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Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (10)
Sample
Human Cell lysate - whole cell (Prostate, Kidney)
Specification
Prostate, Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Nov 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Embryonic stem cell)
Loading amount
100 µg
Specification
Embryonic stem cell
Gel Running Conditions
Reduced Denaturing (8% page gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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