製品の概要

  • 製品名Anti-Thymine Dimer antibody [H3]
  • 製品の詳細
    Mouse monoclonal [H3] to Thymine Dimer
  • 特異性Monoclonal Anti-Thymine Dimer reacts specifically with the (5’-6’) cyclobutane type of homothymine or thymine-cytosine heterodimers. The antibody reacts with thymine dimers in single-stranded DNA, and has a lower affinity for the dimer in short oligonucleotides (a tail of minimum 10-20 thymine residues is required for efficient labeling of oligonucleotide probes).
  • アプリケーション適用あり: Southern Blot, ICC, Competitive ELISA, ELISA, ICC/IFmore details
  • 免疫原

    Chemical/ Small Molecule.

  • 特記事項


    Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.
  • ポリ/モノモノクローナル
  • クローン名H3
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Southern Blot Use a concentration of 0.5 - 1 µg/ml.
ICC Use at an assay dependent dilution.
Competitive ELISA Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

Anti-Thymine Dimer antibody [H3] 画像

  • ab10347 staining Thymine Dimer in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permabilized using 0.5% Triton X-100, blocked with 5% BSA for 15 minutes at 20°C, then incubated with ab10347 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated rabbit anti mouse polyclonal, used at a 1/500 dilution.

    See Abreview

Anti-Thymine Dimer antibody [H3] (ab10347) 使用論文

This product has been referenced in:
  • Moriel-Carretero M & Aguilera A A Postincision-Deficient TFIIH Causes Replication Fork Breakage and Uncovers Alternative Rad51- or Pol32-Mediated Restart Mechanisms. Mol Cell 37:690-701 (2010). Read more (PubMed: 20227372) »
  • Parro V  et al. SOLID2: an antibody array-based life-detector instrument in a Mars Drilling Simulation Experiment (MARTE). Astrobiology 8:987-99 (2008). ELISA . Read more (PubMed: 19105755) »

See all 2 Publications for this product

Product Wall

Apologies, the expiration date should have been 30th September 2012. I have updated our records to reflect the correct date.

Please let me know if there is anything else I can help you with.

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (U2OS)
Specification U2OS
Permeabilization Yes - triton-X 100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (u2os)
Specification u2os
Type Direct
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Andrew Cobb

Verified customer

投稿 Jul 16 2013

Thank you for your message.

I can confirm that Thymine Dimer is not a species specific target, and so this antibody should work in pig.

As far as we are aware, ab10347 has never been tested in IHC-P. All tested applications covere...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Whole skin)
Specification Whole skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - 0.1% Tween-20
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 11 2012

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

Having reviewed the protocol details, and after all the optimisation attempts, I am afraid ...

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I am sending you the new discount codes below, but would suggest if you encounter problems with using the codes for an online order, please give us a call so thatCustomer Service can help you with applying the codes to the order.

DISCOUNT COD...

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Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Acetone
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Jul 06 2012

Thank you for your reply.

I apologize for this oversight on our end, and I am resending you new codes that should work for you.

DISCOUNT CODE: xxx for ab10347
DISCOUNT CODE: xxx for ab23722
EXPIRATION: xxx

Than...

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Thank you for contacting us.

Yes, I checked and the codes that were sent to you seem to have expired:

ab23722 : DISCOUNT CODE: xxx
Expiration date: xxx

ab10347: DISCOUNT CODE: xxx
Expiration date: xxx
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"