Anti-Thymidylate Synthase 抗体 [SP112] (ab111153)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP112] to Thymidylate Synthase
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Thymidylate Synthase antibody [SP112]
Thymidylate Synthase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP112] to Thymidylate Synthase -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Colon carcinoma and tonsil tissue, human hepatocellular carcinoma. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
SP112 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111153の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/200.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/200. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. -
パスウェイ
Pyrimidine metabolism; dTTP biosynthesis. -
配列類似性
Belongs to the thymidylate synthase family. -
細胞内局在
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7298 Human
- Omim: 188350 Human
- SwissProt: P04818 Human
- Unigene: 369762 Human
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別名
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling Thymidylate Synthase with ab111153 at 1/100 dilution (1.55 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human hepatocellular carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab111153 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Thymidylate Synthase with ab111153 at 1/100 dilution (1.55 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, with some nuclear staining, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab111153 for 30 mins at room temperature. -
Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Thymidylate Synthase with purified ab111153 at 1/200 dilution (0.78 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab111153 は論文での使用が確認できていません。