製品の概要

  • 製品名Anti-Thymidylate Synthase antibody
    Thymidylate Synthase 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Thymidylate Synthase
  • アプリケーション適用あり: ELISA, IP, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Escherichia coli , African Green Monkey
    非交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

  • ポジティブ・コントロール
    • HeLa cells treated with the TS inhibitor 5-FUdR
  • 特記事項The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Pre-clearing with a non-specific sheep IgG is helpful to reduce background.

IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.

ターゲット情報

Anti-Thymidylate Synthase antibody 画像



  • Predicted band size : 37 kDa
  • IHC image of Thymidylate Synthase staining in Human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7398, 5µg/ml, for 15 mins at room temperature. A Rabbit anti-Sheep biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Thymidylate Synthase antibody (ab7398) 使用論文

This product has been referenced in:
  • MacFarlane AJ  et al. Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity. J Nutr 144:419-24 (2014). Read more (PubMed: 24500934) »
  • Macfarlane AJ  et al. Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA. J Biol Chem 286:44015-22 (2011). WB ; Mouse . Read more (PubMed: 22057276) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. For ab7398 and 3145 - you can use 5-FU-resistant cancer cell lines or human breast, colorectal, gastric, head and neck carcinomas as positive controls. All the cells especially the rapidly proliferating cells express this...

Read More

This is a polyclonal antibody, and so we cannot refer to subclasses. The concentration is 10mg/ml and the sensitivity can be seen from the recommended concentrations for each application.

This antibody was raised against the full length recombinant protien. The epitopes recognised by this antibody have not been mapped.

This antibody was raised against a recombinant protein. A carrier protein was not used for immunisation.

The antibody is an IgG fraction. Unfortunatly we do not have an example to hand of the Western blot. However, the antibody works very well in Western blotting and we recommend this antibody for this application.

Yes, the anti-hTS does work on paraffin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"