製品の概要

  • 製品名Anti-Thymidylate Synthase antibody
    Thymidylate Synthase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Thymidylate Synthase
  • アプリケーション適用あり: Flow Cyt, WB, IHC-P, ICC/IF, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC , corresponding to amino acids 111-211 of Human Thymidylate Synthase

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
ICC Use at an assay dependent concentration. PubMed: 23840376

ターゲット情報

  • 機能Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
  • パスウェイPyrimidine metabolism; dTTP biosynthesis.
  • 配列類似性Belongs to the thymidylate synthase family.
  • 細胞内局在Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • d TMP synthase antibody
    • EC 2.1.1.45 antibody
    • HsT422 antibody
    • MGC88736 antibody
    • OTTHUMP00000162195 antibody
    • Thymidylate synthase antibody
    • Thymidylate synthetase antibody
    • TMS antibody
    • TS antibody
    • TSase antibody
    • Tyms antibody
    • TYMS protein antibody
    • Tyms thymidylate synthetase antibody
    • TYSY_HUMAN antibody
    see all

Anti-Thymidylate Synthase antibody 画像

  • Thymidylate Synthase antibody (ab58287) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 36 kDa
    Thymidylate Synthase antibody (ab58287) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab58287 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58287, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Thymidylate Synthase antibody (ab58287) at 1 µg/ml

    Lane 1 : Recombinant Human Thymidylate Synthase protein (ab95378) at 0.1 µg
    Lane 2 : Recombinant Human Thymidylate Synthase protein (ab95378) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • Overlay histogram showing HeLa cells stained with ab58287 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58287, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Thymidylate Synthase antibody (ab58287) 使用論文

This product has been referenced in:
  • Del Bufalo D  et al. Histone deacetylase inhibition synergistically enhances pemetrexed cytotoxicity through induction of apoptosis and autophagy in non-small cell lung cancer. Mol Cancer 13:230 (2014). WB ; Human . Read more (PubMed: 25301686) »
  • Szulkin A  et al. Variation in drug sensitivity of malignant mesothelioma cell lines with substantial effects of selenite and bortezomib, highlights need for individualized therapy. PLoS One 8:e65903 (2013). ICC ; Human . Read more (PubMed: 23840376) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab58287.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"