製品の概要

  • 製品名Anti-Thymidylate Synthase antibody
    Thymidylate Synthase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Thymidylate Synthase
  • 特異性This antibody recognizes Thymidylate Synthase and an additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. (This event occurs in most human breast, colorectal, gastric, head and neck carcinomas.) The antibody can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
  • アプリケーション適用あり: ELISA, IP, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, African Green Monkey
  • 免疫原

    Recombinant full length protein (Human) expressed in E. coli.

  • ポジティブ・コントロール
    • HeLa cell extracts.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab34836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/100000.
IP 1/500.
WB 1/1000. Predicted molecular weight: 36 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

ターゲット情報

Anti-Thymidylate Synthase antibody 画像

  • ICC/IF image of ab34836 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Thymidylate Synthase antibody (ab34836) 使用論文

ab34836 has not yet been referenced specifically in any publications.

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