製品の概要

  • 製品名
    Anti-Thymidine Kinase 1 antibody
    Thymidine Kinase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Thymidine Kinase 1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 205 and 234 of human Thymidine Kinase (NP_003249.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 配列類似性
    Belongs to the thymidine kinase family.
  • 翻訳後修飾
    Phosphorylated on Ser-13 in mitosis.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

画像

  • All lanes : Anti-Thymidine Kinase 1 antibody (ab75277) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 130 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
    ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

参考文献

ab75277 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab75277.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録