製品の概要
- 製品名Anti-Thymidine Kinase 1 antibody
Thymidine Kinase 1 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to Thymidine Kinase 1
- アプリケーション適用あり: WB, IPmore details
- 種交差性交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
- 免疫原
Synthetic peptide corresponding to a region between residues 205 and 234 of human Thymidine Kinase (NP_003249.1)
- ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- バッファーPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... - 精製度Immunogen affinity purified
- 特記事項（精製）ab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support.
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
- Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab75277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
- 配列類似性Belongs to the thymidine kinase family.
- 翻訳後修飾Phosphorylated on Ser-13 in mitosis.
- 細胞内局在Cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 7083 Human
- Omim: 188300 Human
- SwissProt: P04183 Human
- Unigene: 515122 Human
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別名
- cytosolic antibody
- KITH_HUMAN antibody
- Thymidine kinase 1 antibody
see all
Anti-Thymidine Kinase 1 antibody 画像
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Western blot - Thymidine Kinase antibody (ab75277)All lanes : Anti-Thymidine Kinase 1 antibody (ab75277) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 25 kDa
Observed band size : 25 kDa
Additional bands at : 130 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds -
Immunoprecipitation - Thymidine Kinase antibody (ab75277)Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Anti-Thymidine Kinase 1 antibody (ab75277) 使用論文
ab75277 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"