製品の概要
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製品名Anti-Thymidine Kinase 1 antibody
Thymidine Kinase 1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Thymidine Kinase 1
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由来種Rabbit
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 205 and 234 of human Thymidine Kinase (NP_003249.1)
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ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab75277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
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配列類似性Belongs to the thymidine kinase family.
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翻訳後修飾Phosphorylated on Ser-13 in mitosis.
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細胞内局在Cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 7083 Human
- Omim: 188300 Human
- SwissProt: P04183 Human
- Unigene: 515122 Human
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別名
- cytosolic antibody
- KITH_HUMAN antibody
- Thymidine kinase 1 antibody
see all
画像
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Western blot - Anti-Thymidine Kinase 1 antibody (ab75277)All lanes : Anti-Thymidine Kinase 1 antibody (ab75277) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 130 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunoprecipitation - Anti-Thymidine Kinase 1 antibody (ab75277)Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
参考文献
ab75277 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"