製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to THRSP
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human THRSP.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse Liver tissue lysate. IHC: Human breast adipose frozen tissue sections.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-Fr Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Plays a role in the regulation of lipogenesis, especially in lactating mammary gland. Important for the biosynthesis of triglycerides with medium-length fatty acid chains. May modulate lipogenesis by interacting with MID1IP1 and preventing its interaction with ACACA (By similarity). May function as transcriptional coactivator. May modulate the transcription factor activity of THRB.
  • 組織特異性
    Mainly expressed in tissues that synthesize triglycerides.
  • 配列類似性
    Belongs to the SPOT14 family.
  • 細胞内局在
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Lpgp antibody
    • LPGP1 antibody
    • MGC21659 antibody
    • OTTHUMP00000233866 antibody
    • S14 antibody
    • S14 protein antibody
    • SPOT 14 antibody
    • Spot 14 protein antibody
    • SPOT14 antibody
    • SPOT14 homolog antibody
    • Thrsp antibody
    • THRSP_HUMAN antibody
    • Thyroid hormone inducible hepatic protein antibody
    • Thyroid hormone responsive (SPOT14 homolog rat) antibody
    • Thyroid hormone responsive antibody
    • Thyroid hormone responsive protein antibody
    • Thyroid hormone responsive SPOT14 antibody
    • Thyroid hormone-inducible hepatic protein antibody
    see all

Anti-THRSP antibody 画像

  • IHC image of THRSP staining in human breast adipose frozen tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97504, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-THRSP antibody (ab97504) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab97504 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97504 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%, 5min) Hek293, HepG2, and MCF-7 cells at 1ug/ml.

Anti-THRSP antibody (ab97504) 使用論文

This product has been referenced in:
  • Wellberg EA  et al. Modulation of tumor fatty acids, through overexpression or loss of thyroid hormone responsive protein spot 14 is associated with altered growth and metastasis. Breast Cancer Res 16:481 (2014). WB ; Mouse . Read more (PubMed: 25472762) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (gel 15%)
Sample
Cow Cell lysate - whole cell (Bovine mammary epithelial cells)
Specification
Bovine mammary epithelial cells
Treatment
150nM tran10,cis12 CLA for 24hrs
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Miss. xinbei huang

Verified customer

投稿 Aug 13 2014

Thank you for contacting us.

The lot we have currently in stock for product ab97504 is XXXXXXX.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for following this up with the customer.

I am really sorry, however I do really not know what the problem is. I would expect nice bands. Unfortunately we do not have an alternative antibody in our catalogue anymore.
<...

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Thank you for sending this information and for your patience.

I am sorry that the customer is experiencing problems also with this antibody.In order to exclude any technical problem, I would like to raise the two following points:

1...

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Thank you very much for your help resolving this case and for the answers from the customer. Indeed, I agree that in the results there are too many bands to make a confident decision of which one is the correct band. As only last suggestion I c...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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