製品の概要

  • 製品名Anti-Thrombospondin antibody [A6.1] (Biotin)
    Thrombospondin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [A6.1] to Thrombospondin (Biotin)
  • 標識Biotin
  • 特異性ab79450 shows no cross reaction with fibronectin, fibrinogen, and von Willebrand factor. It shows a mild cross reaction with Thrombospondin 2.
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, Electron Microscopy, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Horse, Cow, Dog, Human, Pig
  • 免疫原

    Reduced and alkylated purified human Thrombospondin (fully denatured) from the supernatant of thrombin activated platelets

  • エピトープCollagen Type V binding domain of Thrombospondin
  • ポジティブ・コントロール
    • Platelets in placenta, megakaryocytes in bone marrow and tonsil
  • 特記事項


    ab79450 inhibits Thrombospondin collagen interaction. Its binding to Thrombospondin is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate, and heparin). Its binding is enhanced by EDTA i.e. at low concentration of Ca2+.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 130 kDa.

For 2 hours at room temperature.

IP Use at 2 µg/mg of lysate.
IHC-P 1/25 - 1/50.
Flow Cyt Use at an assay dependent concentration. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Electron Microscopy Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

Anti-Thrombospondin antibody [A6.1] (Biotin) (ab79450) 使用論文

This product has been referenced in:
  • Galvin NJ  et al. Interaction of human thrombospondin with types I-V collagen: direct binding and electron microscopy. J Cell Biol 104:1413-22 (1987). Read more (PubMed: 3571333) »
  • Dixit VM  et al. Monoclonal antibodies that recognize calcium-dependent structures of human thrombospondin. Characterization and mapping of their epitopes. J Biol Chem 261:1962-8 (1986). Read more (PubMed: 2418018) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"