製品の概要

  • 製品名
    Anti-Thrombospondin antibody [A6.1]
    Thrombospondin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [A6.1] to Thrombospondin
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, IHC-P, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Horse, Cow, Dog, Human, Pig
  • 免疫原

    BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

  • ポジティブ・コントロール
    • Tonsil

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

Anti-Thrombospondin antibody [A6.1] 画像

  • Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.

  • Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody.

    See Abreview

  • ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .

    This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.

    See Abreview

Anti-Thrombospondin antibody [A6.1] (ab1823) 使用論文

This product has been referenced in:
  • Shearer JJ  et al. Inorganic Arsenic-Related Changes in the Stromal Tumor Microenvironment in a Prostate Cancer Cell-Conditioned Media Model. Environ Health Perspect 124:1009-15 (2016). WB ; Human . Read more (PubMed: 26588813) »
  • Labrousse-Arias D  et al. HIF-2a-mediated induction of pulmonary thrombospondin-1 contributes to hypoxia-driven vascular remodelling and vasoconstriction. Cardiovasc Res 109:115-30 (2016). Human . Read more (PubMed: 26503986) »

See all 29 Publications for this product

Product Wall

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (4-20% mini gel)
Sample
Mouse Tissue lysate - whole (Penile Tissue)
Specification
Penile Tissue
Blocking step
SuperBlock Blocking Buffer (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. Gwen Lagoda

Verified customer

投稿 Nov 11 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Pig Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 29 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Rabbit Tissue sections (splen rabbit)
Specification
splen rabbit
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Rat Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 04 2013


There are a number of tissues that could serve as positive controls. We do not have sections of mouse tonsil, which is listed on the datasheet as a positive control, but sections of spleen (ab4613) or placenta (ab4612) are also good choices for IH...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEFs)
Specification
MEFs
Fixative
Formaldehyde
Permeabilization
Yes - TBS Tw20
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Dec 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (1BRG3 fibroblast)
Specification
1BRG3 fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - Tween 20
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 12 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Meriones unguiculatus Tissue sections (spleen)
Specification
spleen
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Andrea Dannullis

Verified customer

投稿 Sep 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (intestinum)
Specification
intestinum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - PBS/TritonX
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsille)
Specification
tonsille
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 18 2012

1-10 of 43 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録