Anti-Thrombospondin 2 抗体 (ab84469)

製品の概要

  • 製品名
    Anti-Thrombospondin 2 antibody
    Thrombospondin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Thrombospondin 2
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Thrombospondin 2.

    (Peptide available as ab96113.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human and Mouse spinal cord tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

画像

  • All lanes : Anti-Thrombospondin 2 antibody (ab84469) at 1 mg/ml

    Lane 1 : Mouse spinal cord embryo E14
    Lane 2 : Human skin
    Lane 3 : Mouse skin
    Lane 4 : A431 (Human epithelial carcinoma cell line)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 129 kDa
    Additional bands at : 210 kDa (possible glycosylated form),46 kDa (possible cleavage fragment),58 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    ab84469 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

  • ICC/IF image of ab84469 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86649, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab84469 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録