Anti-Thrombospondin 2 抗体 (ab84469)
Key features and details
- Rabbit polyclonal to Thrombospondin 2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Thrombospondin 2 antibody
Thrombospondin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Thrombospondin 2 -
由来種
Rabbit -
特異性
Reactivity in Rat is no longer guaranteed due to difficulty reproducing in-house experimental evidence, but will remain predicted, due to the immunogen showing 100% xr with Rat. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Chicken, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human Thrombospondin 2 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available asab96113) -
ポジティブ・コントロール
- This antibody gave a positive signal in both Human and Mouse spinal cord tissue lysate. This antibody gave a positive signal in ICC/IF in U2O2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84469の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. -
組織特異性
High expression in invertebral disk tissue. -
関連疾患
Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. -
配列類似性
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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参照データベース
- Entrez Gene: 414837 Chicken
- Entrez Gene: 338092 Cow
- Entrez Gene: 7058 Human
- Entrez Gene: 21826 Mouse
- Entrez Gene: 292406 Rat
- Omim: 188061 Human
- SwissProt: P35440 Chicken
- SwissProt: Q95116 Cow
see all -
別名
- THBS2 antibody
- Thrombospondin-2 antibody
- TSP2 antibody
- TSP2_HUMAN antibody
画像
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Ab84469 staining Thrombospondin 2 in U2O2 cells. The cells were fixed with 100% Methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab84469 at 10ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and tubulin was labelled using ab7291 Mouse monoclonal to alpha Tubulin together with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594), at a 1/1000 dilution (shown in red).
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All lanes : Anti-Thrombospondin 2 antibody (ab84469) at 1 mg/ml
Lane 1 : Mouse spinal cord embryo E14
Lane 2 : Human skin
Lane 3 : Mouse skin
Lane 4 : A431 (Human epithelial carcinoma cell line)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Additional bands at: 210 kDa (possible glycosylated form), 46 kDa (possible cleavage fragment), 58 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab84469 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab84469 は 11 報の論文で使用されています。
- Li Y et al. Circ-OPHN1 suppresses the proliferation, migration, and invasion of trophoblast cells through mediating miR-558/THBS2 axis. Drug Dev Res 83:1034-1046 (2022). PubMed: 35277867
- Liu J et al. circ_0085296 inhibits the biological functions of trophoblast cells to promote the progression of preeclampsia via the miR-942-5p/THBS2 network. Open Med (Wars) 17:577-588 (2022). PubMed: 35415249
- Hu T et al. Astrocyte derived TSP2 contributes to synaptic alteration and visual dysfunction in retinal ischemia/reperfusion injury. Cell Biosci 12:196 (2022). PubMed: 36471420
- Ye Y et al. Circ-AK2 is associated with preeclampsia and regulates biological behaviors of trophoblast cells through miR-454-3p/THBS2. Placenta 103:156-163 (2021). PubMed: 33129036
- Rashid MU et al. Zika virus dysregulates human Sertoli cell proteins involved in spermatogenesis with little effect on tight junctions. PLoS Negl Trop Dis 14:e0008335 (2020). PubMed: 32511241