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Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Thrombospondin 2.
(Peptide available as ab96113.)
Our Abpromise guarantee covers the use of ab84469 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab84469 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab84469 has not yet been referenced specifically in any publications.