Anti-Thrombopoietin 抗体 (ab93909)


  • 製品名Anti-Thrombopoietin antibody
    Thrombopoietin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Thrombopoietin
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Thrombopoietin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human liver tissue lysate.



Our Abpromise guarantee covers the use of ab93909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 38 kDa).


  • 機能Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • 関連疾患Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
  • 配列類似性Belongs to the EPO/TPO family.
  • ドメインTwo-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C mpl ligand antibody
    • C-mpl ligand antibody
    • Megakaryocyte colony stimulating factor antibody
    • Megakaryocyte colony-stimulating factor antibody
    • Megakaryocyte growth and development factor antibody
    • Megakaryocyte stimulating factor antibody
    • MGC163194 antibody
    • MGDF antibody
    • MKCSF antibody
    • ML antibody
    • MPL ligand antibody
    • MPLLG antibody
    • Myeloproliferative leukemia virus oncogene ligand antibody
    • Prepro thrombopoietin antibody
    • THCYT1 antibody
    • THPO antibody
    • Thrombopoietin antibody
    • Thrombopoietin nirs variant 1 antibody
    • TPO antibody
    • TPO_HUMAN antibody
    see all

Anti-Thrombopoietin antibody 画像

  • Anti-Thrombopoietin antibody (ab93909) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute
  • ICC/IF image of ab93909 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93909 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Thrombopoietin antibody (ab93909) 使用論文

ab93909 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cultured Cells (embryonic stem cells)
Specification embryonic stem cells
Permeabilization No
Fixative Methanol

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投稿 Jun 07 2013

Thank you for contacting us.

Your credit note ID for the refund of both antibodies is xxx.

I am sorry that both antibodies did not perform as stated on their datasheets. I have asked our Finance department to issue a credit note for y...

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Thank you for your reply. I would still recommend checking if the antibodies bind to the recombinant protein in a dot blot assay. Because thrombopoietin is a secreted protein, it is very possible that it may be washed away from your cells during the st...

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Thank you for your reply. If I am understanding your assay correctly, your cells are overexpressing a thrombopoietin receptor and you are incubating the cell culture with recombinant thrombopoietin to measure the binding efficacy. What type of recombin...

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Thank you for your reply and the additional information.

Regarding the refund, you would need to let your purchasing department know the information I had sent and they then should contact us so that the refund can be issued to you. I'm certai...

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Thank you for contacting us.
I am sorry to hear that these antibodies do not work as expected.

According to the records in our system, the purchase of ab40664 has already been refunded and the case has been discussed and resolved.

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Thank you for your reply.

Only ab93909 has been tested with cultured cells (ICC/IF), while ab128487 has not yet been tested in this application. I can sendyou retroactively a testing discount code for trying it.

For UNTESTED specie...

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