製品の概要

  • 製品名Anti-Thrombin Receptor antibody
    Thrombin Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Thrombin Receptor
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Baboon
  • 免疫原

    Synthetic peptide:

    SFLLRNPND KYEPF

    , corresponding to amino acids 42-55 of Human Thrombin Receptor

  • ポジティブ・コントロール
    • KNRK cells, Pancreatic tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
  • 組織特異性Platelets and vascular endothelial cells.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 翻訳後修飾A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
    Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CF2R antibody
    • Coagulation factor II (thrombin) receptor antibody
    • Coagulation factor II receptor antibody
    • F2R antibody
    • HTR antibody
    • PAR 1 antibody
    • PAR-1 antibody
    • PAR1 antibody
    • PAR1_HUMAN antibody
    • Protease activated receptor 1 antibody
    • Proteinase activated receptor 1 antibody
    • Proteinase-activated receptor 1 antibody
    • Thrombin receptor antibody
    • TR antibody
    see all

Anti-Thrombin Receptor antibody 画像

  • This image shows human pancreas stained with ab32611.
  • Anti-Thrombin Receptor antibody (ab32611) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 87 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-Thrombin Receptor antibody (ab32611) 使用論文

This product has been referenced in:
  • Chen KD  et al. Interconnections between autophagy and the coagulation cascade in hepatocellular carcinoma. Cell Death Dis 5:e1244 (2014). WB ; Human . Read more (PubMed: 24853422) »
  • Aytekin M  et al. Abnormal platelet aggregation in idiopathic pulmonary arterial hypertension: role of nitric oxide. Am J Physiol Lung Cell Mol Physiol 302:L512-20 (2012). WB ; Human . Read more (PubMed: 22246002) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us in regards to the Anti-Thrombin Receptor antibody (ab32611) and sorry for the late reply.

As discussed over the phone, I have enquired as to whether we are able to provide the immunising peptide used to raise the a...

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Thank you for contacting us.

The Uniprot http://www.uniprot.org/uniprot/P25116 database shows, the active part of protein "human Proteinase-activated receptor 1" has amino acid sequence between 42-425, so antibodies specific to this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"