Anti-Thrombin 抗体 [BDI905] (ab20877)


  • 製品名
    Anti-Thrombin antibody [BDI905]
    Thrombin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BDI905] to Thrombin
  • 由来種
  • 特異性
    Ab20877 has not been tested for cross-reactivity with prothrombin therefore we do not know if it will cross react with it.
  • アプリケーション
    適用あり: ICC/IF, ELISAmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (purified) (Human)



Our Abpromise guarantee covers the use of ab20877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent dilution.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
    • 組織特異性
      Expressed by the liver and secreted in plasma.
    • 関連疾患
      Factor II deficiency
      Ischemic stroke
      Thrombophilia due to thrombin defect
      Pregnancy loss, recurrent, 2
    • 配列類似性
      Belongs to the peptidase S1 family.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.
    • 翻訳後修飾
      The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
      N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
    • 細胞内局在
      Secreted, extracellular space.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Coagulation factor II antibody
      • Coagulation factor II thrombin antibody
      • EC antibody
      • F2 antibody
      • Factor II antibody
      • Prepro coagulation factor II antibody
      • Prothrombin antibody
      • Prothrombin B chain antibody
      • PT antibody
      • RPRGL2 antibody
      • Serine protease antibody
      • THPH1 antibody
      • THRB_HUMAN antibody
      • Thrombin heavy chain antibody
      see all


    • ICC/IF image of ab20877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20877, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-goat mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.


    ab20877 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Since I do not know what application the antibody is used in, I am providing a list of possible secondary antibodies.
    Please choose an antibody that is suitable for the planned application. If you would like some help with this, please let me...

    Read More

    Thank you for your enquiry. The isolelectric points and the percentage of glycosylation have not, to our knowledge,been determined for the three antibodies you asked about (ab35799, ab20626, and, in a separate e-mail, ab20877). These are all IgG1 isoty...

    Read More