Anti-Thrombin 抗体 [BDI905] (ab20877)


  • 製品名Anti-Thrombin antibody [BDI905]
    Thrombin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BDI905] to Thrombin
  • 特異性Ab20877 has not been tested for cross-reactivity with prothrombin therefore we do not know if it will cross react with it.
  • アプリケーション適用あり: ICC/IF, ELISAmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (purified) (Human)



Our Abpromise guarantee covers the use of ab20877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent dilution.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 関連性Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. The molecular mass of thrombin is 37 kDa.
    • 細胞内局在Secreted
    • 参照データベース
    • 別名
      • Coagulation factor II antibody
      • Coagulation factor II thrombin antibody
      • EC antibody
      • F2 antibody
      • Factor II antibody
      • Prothrombin B chain antibody
      • PT antibody
      • Serine protease antibody
      see all

    Anti-Thrombin antibody [BDI905] 画像

    • ICC/IF image of ab20877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20877, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-goat mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

    Anti-Thrombin antibody [BDI905] (ab20877) 使用論文

    ab20877 has not yet been referenced specifically in any publications.

    Product Wall

    Since I do not know what application the antibody is used in, I am providing a list of possible secondary antibodies.
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