Anti-ThPok 抗体 (ab70991)

製品の概要

  • 製品名Anti-ThPok antibody
    ThPok 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ThPok
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    KLH conjugated synthetic peptide selected from the center region of human ThPok

  • ポジティブ・コントロール
    • Mouse brain tissue lysates, human hepatocarcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.
  • 配列類似性Contains 1 BTB (POZ) domain.
    Contains 4 C2H2-type zinc fingers.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c Krox antibody
    • cKrox antibody
    • DKFZp686G01254 antibody
    • hc Krox antibody
    • hcKrox antibody
    • Krueppel-related zinc finger protein cKrox antibody
    • Kruppel related zinc finger protein c Krox antibody
    • Kruppel related zinc finger protein cKrox antibody
    • Kruppel related zinc finger protein hcKrox antibody
    • T helper inducing POZ/Kruppel like factor antibody
    • T-helper-inducing POZ/Krueppel-like factor antibody
    • Th-POK antibody
    • ThPOK antibody
    • ZBT7B_HUMAN antibody
    • ZBTB 15 antibody
    • ZBTB 7B antibody
    • ZBTB15 antibody
    • ZBTB7B antibody
    • ZFP 67 antibody
    • Zfp-67 antibody
    • ZFP67 antibody
    • Zinc finger and BTB domain containing 15 antibody
    • Zinc finger and BTB domain containing 7B antibody
    • Zinc finger and BTB domain containing protein 15 antibody
    • Zinc finger and BTB domain containing protein 7B antibody
    • Zinc finger and BTB domain-containing protein 15 antibody
    • Zinc finger and BTB domain-containing protein 7B antibody
    • Zinc finger protein 67 homolog antibody
    • Zinc finger protein 857B antibody
    • Zinc finger protein Th POK antibody
    • Zinc finger protein Th-POK antibody
    • Zinc finger protein ThPOK antibody
    • ZNF857B antibody
    see all

Anti-ThPok antibody 画像

  • Anti-ThPok antibody (ab70991) at 1/60 dilution + mouse brain tissue lysates at 35 µg

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 33 kDa,72 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with 1/50 ab70991. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

Anti-ThPok antibody (ab70991) 使用論文

ab70991 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - nuclear (WT thymocyte)
Specification WT thymocyte
Negative control Actb-F: ACTCCTGCTTGCTGATCCAC Actb-R: CTTAGCTTGGTGAGGGTGGG
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Paraformaldehyde
Positive control CD8 primers from Rui et al., J Immunol 2012 189:1380-1390 CD8-1-F: CACCAACCAAGAAGACTACC CD8-1-R: CTAGAGAAATAGGCTACAGAGC CD8-2-F: GTCTGGTCTACACAGTGAGGA CD8-2-R: GCTGATCTGAGGGACTATTG IgH/Cyp3a primers from Zullo et al., Cell 2012 149:1474-1487 IgH-1-F: CAACAAATACATGAAATTACACACCG IgH-1-R: TTTTATATCCAGCTACTTAACTAAACCTGG IgH-2-F: GGGAGCCCATGACCAGC IgH-2-R: CATGTTAATCACCACATTGCAGATC Cyp3a-1-F: GCTTTAAAGATTCAAAAGGATGGTACC Cyp3a-1-R: ACATGGTGGCAGGAACAG
Username

Abcam user community

Verified customer

投稿 Dec 08 2014

The better choice will be ab70991, as the immunogen is 100% conserved in rat ThPok. The immunogen for ab23388 is conserved 85%, so reactivity is predicted, but is less probable than ab70991 reactivity.

Thank you for sending this information.

If you would like, I can issue free of charge replacements of the antibody and lysate with another anti-ThPok antibody such as ab23388 or ab70991 and our recommended positive control lysate. Like ab9...

Read More

Thank you for your enquiry.

I am sorry to confirm that non of our thPok antibodies have been tested and guaranteed in Xenopus tropicalis. I have checked the alignment of the sequence you kindly provided with the immunogen sequences for the a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"