Anti-ThPok 抗体 (ab23388)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ThPok
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide, derived from a region between residues 291-345 of Human ThPok.

  • ポジティブ・コントロール
    • HepG2 cell lysate, Human heart and human lung tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.
  • 配列類似性
    Contains 1 BTB (POZ) domain.
    Contains 4 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c Krox antibody
    • cKrox antibody
    • DKFZp686G01254 antibody
    • hc Krox antibody
    • hcKrox antibody
    • Krueppel-related zinc finger protein cKrox antibody
    • Kruppel related zinc finger protein c Krox antibody
    • Kruppel related zinc finger protein cKrox antibody
    • Kruppel related zinc finger protein hcKrox antibody
    • T helper inducing POZ/Kruppel like factor antibody
    • T-helper-inducing POZ/Krueppel-like factor antibody
    • Th-POK antibody
    • ThPOK antibody
    • ZBT7B_HUMAN antibody
    • ZBTB 15 antibody
    • ZBTB 7B antibody
    • ZBTB15 antibody
    • ZBTB7B antibody
    • ZFP 67 antibody
    • Zfp-67 antibody
    • ZFP67 antibody
    • Zinc finger and BTB domain containing 15 antibody
    • Zinc finger and BTB domain containing 7B antibody
    • Zinc finger and BTB domain containing protein 15 antibody
    • Zinc finger and BTB domain containing protein 7B antibody
    • Zinc finger and BTB domain-containing protein 15 antibody
    • Zinc finger and BTB domain-containing protein 7B antibody
    • Zinc finger protein 67 homolog antibody
    • Zinc finger protein 857B antibody
    • Zinc finger protein Th POK antibody
    • Zinc finger protein Th-POK antibody
    • Zinc finger protein ThPOK antibody
    • ZNF857B antibody
    see all

画像



  • Predicted band size : 60 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling ThPok with ab23388 at 1.0µg/ml.
  • Anti-ThPok antibody (ab23388) + HepG2 cell lysate (20ug)

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,000.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    ab23388 titration 0.004ug/ml.
  • Immunohistochemical analysis of ThPok expression in paraffin embedded human heart tissue section. ab23388 was used at 4 ug/ml. Arrows indicate the positively labelled myocardial cells.

  • Immunohistochemical analysis of ThPok expression in paraffin embedded human lung tissue section. ab23388 was used at 4 ug/ml. Arrows indicate the positively labelled alveolar cells.

  • All lanes : Anti-ThPok antibody (ab23388) at 1.25 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 60 kDa

参考文献

ab23388 has not yet been referenced specifically in any publications.

レビューと Q&A

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Abreviews
Application
Flow Cytometry
Fixation
eBioscience transcription factor fixation buffer
Permeabilization
Yes - eBioscience transcription factor permeabilization buffer
Sample
Human Cell (Thymocytes and PBMCs)
Specification
Thymocytes and PBMCs
Gating Strategy
Lymphocytes - doublet exclusion - CD4+ vs. CD8+
Preparation
Cell harvesting/tissue preparation method: meshed/Ficoll
Sample buffer: PBS/BSA
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投稿 Jun 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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