Anti-Thomsen-Friedenreich Antigen 抗体 [A78-G/A7] (ab80553)

製品の概要

  • 製品名Anti-Thomsen-Friedenreich Antigen antibody [A78-G/A7]
    Thomsen-Friedenreich Antigen 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [A78-G/A7] to Thomsen-Friedenreich Antigen
  • 特異性ab80553 is specific for both anomeric forms of the disaccharide (TFalpha and TFbeta, including related structures on the glycolipid) and shows no cross reactivity with sialylated glycophorin.
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Neuraminidase-treated human red blood cells.

  • エピトープGalß1-3GalNAc
  • ポジティブ・コントロール
    • KG1 cells. Human colorectal carcinoma tissue. Human colon adenocarcinoma tissue.
  • 特記事項


    ab80553 can be applied for the detection of cells with Thomsen-Friedenreich antigen and is especially applicable for sensitive determination of neuraminidases. It can be used on frozen and formalin fixed, paraffin fixed tissues and is capable of agglutinating desialylated red blood cells (Karsten et al, 1995).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • 特記事項(精製)Purified from the ascites fluid by ammonium sulfate precipitation.
  • 一次抗体 備考ab80553 can be applied for the detection of cells with Thomsen-Friedenreich antigen and is especially applicable for sensitive determination of neuraminidases. It can be used on frozen and formalin fixed, paraffin fixed tissues and is capable of agglutinating desialylated red blood cells (Karsten et al, 1995).
  • ポリ/モノモノクローナル
  • クローン名A78-G/A7
  • アイソタイプIgM
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

1/25 - 1/50 for 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性The Thomsen Friedenreich antigen acts as an oncofoetal antigen, with low expression in normal adult tissues but increasing to foetal levels of expression in hyperplasia or malignancy. It is considered as a pan carcinoma marker. Can be applied for the detection of cells with TF antigen and is especially applicable for sensitive determination of neuraminidases.
  • 細胞内局在Cell Membrane
  • 別名
    • CD176 antibody
    • TF antibody
    • TF antigen antibody

Anti-Thomsen-Friedenreich Antigen antibody [A78-G/A7] (ab80553) 使用論文

This product has been referenced in:
  • Baldus SE  et al. Coexpression of MUC1 mucin peptide core and the Thomsen-Friedenreich antigen in colorectal neoplasms. Cancer 82:1019-27 (1998). Read more (PubMed: 9506345) »
  • Karsten U  et al. A new monoclonal antibody (A78-G/A7) to the Thomsen-Friedenreich pan-tumor antigen. Hybridoma 14:37-44 (1995). Read more (PubMed: 7539400) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"