製品の概要

  • 製品名Anti-THOC5 antibody [EPR6905(B)]
    THOC5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6905(B)] to THOC5
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human THOC5.

  • ポジティブ・コントロール
    • 293T, HeLa, NIH 3T3 and A549 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解離定数(KD 値) KD = 4.40 x 10 -11 M Learn more about KD
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR6905(B)
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Component the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and ALYREF/THOC4. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.
    • 組織特異性Ubiquitously expressed.
    • 配列類似性Belongs to the THOC5 family.
    • 翻訳後修飾Phosphorylated on tyrosine upon binding to activated CSF1R; which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C22orf19 antibody
      • Chromosome 22 open reading frame 19 antibody
      • Fmip antibody
      • Fms interacting protein antibody
      • fSAP79 antibody
      • Functional spliceosome-associated protein 79 antibody
      • hTREX90 antibody
      • KIAA0983 antibody
      • LOC400922 antibody
      • NF2/meningioma region protein pK1.3 antibody
      • PK1.3 antibody
      • Placental protein 39.2 antibody
      • PP39.2 antibody
      • THO complex 5 antibody
      • THO complex subunit 5 antibody
      • THO complex subunit 5 homolog antibody
      • thoc5 antibody
      • THOC5_HUMAN antibody
      see all

    Anti-THOC5 antibody [EPR6905(B)] 画像

    • All lanes : Anti-THOC5 antibody [EPR6905(B)] (ab124996) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : NIH 3T3 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 79 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-THOC5 antibody [EPR6905(B)] (ab124996) 使用論文

    ab124996 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124996.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"