製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to THOC5
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    A region between residue 25 and 75 of human THOC5 (NP_003669.4)

  • ポジティブ・コントロール
    • 293T cell lysate, NIH3T3 cell lysate and HeLa cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 79 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Component the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and ALYREF/THOC4. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the THOC5 family.
  • 翻訳後修飾
    Phosphorylated on tyrosine upon binding to activated CSF1R; which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C22orf19 antibody
    • Chromosome 22 open reading frame 19 antibody
    • Fmip antibody
    • Fms interacting protein antibody
    • fSAP79 antibody
    • Functional spliceosome-associated protein 79 antibody
    • hTREX90 antibody
    • KIAA0983 antibody
    • LOC400922 antibody
    • NF2/meningioma region protein pK1.3 antibody
    • PK1.3 antibody
    • Placental protein 39.2 antibody
    • PP39.2 antibody
    • THO complex 5 antibody
    • THO complex subunit 5 antibody
    • THO complex subunit 5 homolog antibody
    • thoc5 antibody
    • THOC5_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling THOC5 with ab86074 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-THOC5 antibody (ab86074) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : mouse NIH3T3 cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 79 kDa
    Observed band size : 79 kDa


    Exposure time : 30 seconds
  • Immunoprecipitation/ Western Blot of ab86074: Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded and ab86074 used for IP at 3 µg/mg lysate and 0.4µg/ml for WB. Chemiluminescence with an exposure time of 10 seconds.

参考文献

ab86074 has not yet been referenced specifically in any publications.

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