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Thioredoxin Reductase Assay Kit (ab83463) is a specific assay for detecting Thioredoxin Reductase (TrxR) activity in various samples. TrxR catalyzes the reduction of DTNB (5, 5'-dithiobis (2-nitrobenzoic) acid) to TNB2- (5-thio-2-nitrobenzoic acid) in presence of NADPH, which generates a strong yellow color (ODmax = 412 nm). Other enzymes present in crude biological samples such as glutathione reductase and glutathione peroxidase can also reduce DTNB. In order to measure TrxR-only activity, a TrxR specific inhibitor is used in a separate reaction to determine TrxR specific activity. The difference between total DTNB reduction in the sample and DTNM reduction in the sample in presence of TrxR inhibitor is the value of specific TrxR activity in the sample.
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Thioredoxin reductase (TrxR, EC 188.8.131.52) is a ubiquitous mammalian enzyme that catalyzes the NADPH-dependent reduction of the redox protein thioredoxin, as well as of other endogenous and exogenous compounds such as selenite, lipid hydroperoxides and hydrogen peroxide. TrxR is the only enzyme known to catalyze the reduction of thioredoxin in order to form reduced disulfide bounds in cells, and it is likely that also plays a role in protection against oxidant injury, cell growth and transformation.
|TNB Standard||Brown||1 vial|
|TrxR Assay Buffer||WM||1 x 25ml|
|TrxR Inhibitor||Clear||1 vial|
|TrxR Positive Control||Green||1 vial|
Our Abpromise guarantee covers the use of ab83463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Thioredoxin reductase measured in mouse tissue lysates showing activity (mU) per mg protein of sample tested
Standard curve (colourimetric) : mean of duplicates (+/-SD) with background readings substracted
Thioredoxin reductase measured in cell lysates showing activity (mU) per 1 mln of cells tested